ABSTRACT
<p><b>OBJECTIVE</b>To study the relationship between tissue quantitative distribution and pharmacokinetics of 3H-achyranthes bidentata ecdysterone and the channel-tropism of herbal drugs in mice.</p><p><b>METHOD</b>3H-achyranthes bidentata ecdysterone was used as a tracer agent and injected into mice by the caudal vein. In 36 hours, the contents of the tracer agent of samples involving 9 different tracing phases and organ or tissue were determined in order to observe the dynamic quantitative distribution and excretion and pharmacokinetics of 3H-achyranthes bidentata ecdysterone and to understand the channel-tropism of herbal drugs achyranthes bidentata.</p><p><b>RESULT</b>3H-achyranthes bidentata ecdysterone of same organs in different tracing phases and the contents of 3H-achyranthes bidentata ecdysterone in same tracing phases of different organs were significantly different (P<0.01). 3H-achyranthes bidentata ecdysterone was mainly distributed, in the liver, kidney, adrenal gland, small intestine and lung. The concentration-time profiles of achyranthes bidentata ecdysterone in rats injected into mice by the caudal vein were shown to fit a two-compartment open model with half-lives of (778.65 +/- 12.36) min, the elimination of achyranthes bidentata ecdysterone from plasma was found to be in accord with linear kinetics.</p><p><b>CONCLUSION</b>The above mentioned selective distribution of 3H-achyranthes bidentata ecdysterone basically coincides with the meridian affinity and zang fu selection of the traditional Chinese medicine drug Achyranthes bidentata. This study will provide a scientific basis for the channel-tropism of A. bidentata.</p>
Subject(s)
Animals , Male , Mice , Achyranthes , Chemistry , Drugs, Chinese Herbal , Metabolism , Pharmacokinetics , Ecdysterone , Metabolism , Pharmacokinetics , Isotope Labeling , Meridians , Organ Specificity , Tissue Distribution , Tritium , ChemistryABSTRACT
With transmission electron microscopy,the endoplasmic reticulum of the parathyroid chief cells in normal and cadmium-treated golden hamsters was observed. After 1 month treatment of cadmium, there were dilated and whorl-shaped rough endoplasmic reticulum in the chief cells. After 3 and 6 months treatment of cadmium, there were concentric membranous body and paired endoplasmic reticulum in the chief cells. The mechanism responsible for the morphological change of the endoplasmic reticulum after cadmium treatment and the possible toxicologic effect were discussed.